Machine Learning Establishes Single-Cell Calcium Dynamics as an Early Indicator of Antibiotic Response
Changes in bacterial physiology necessarily precede cell death in response to antibiotics.Herein we investigate the early disruption of Ca2+ homeostasis as a marker for antibiotic response.Using a machine learning framework, we quantify the temporal information encoded in single-cell Ca2+ dynamics.We find Ca2+ dynamics distinguish kanamycin sensiti